var bibbase_data = {"data":"<img src=\"//bibbase.org/img/ajax-loader.gif\" id=\"spinner\"\n  style=\"display: none;\" alt=\"Loading..\" />\n\n<div id=\"bibbase\">\n\n  <script type=\"text/javascript\">\n    var bibbase = {\n      params: {\"bib\":\"https://api.zotero.org/users/3476755/collections/MSX3XAXZ/items?key=qRbAXV8LpLmQTa9GAmUE20g6&format=bibtex&limit=100\",\"jsonp\":\"1\",\"authorFirst\":\"1\",\"hidemenu\":\"true\",\"commas\":\"true\",\"sort\":\"author_short\",\"groupby\":\"\",\"filter\":\"authors:(Inaba|Shiba|稲葉|柴)\",\"host\":\"bibbase.org\"},\n      data: [{\"bibtype\":\"article\",\"type\":\"article\",\"title\":\"Development of an extender solution for short-term sperm storage to promote seed production in alfonsino <i>Beryx splendens</i>\",\"issn\":\"0021-5392, 1349-998X\",\"url\":\"https://www.jstage.jst.go.jp/article/suisan/advpub/0/advpub_22-00062/_article/-char/ja/\",\"doi\":\"10.2331/suisan.22-00062\",\"language\":\"en\",\"urldate\":\"2023-05-10\",\"journal\":\"NIPPON SUISAN GAKKAISHI\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Hasegawa\"],\"firstnames\":[\"Masatoshi\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Inaba\"],\"firstnames\":[\"Kazuo\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Nagakura\"],\"firstnames\":[\"Yasuhiro\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Noda\"],\"firstnames\":[\"Hiroyuki\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kawai\"],\"firstnames\":[\"Noriaki\"],\"suffixes\":[]}],\"year\":\"2023\",\"pages\":\"22–00062\",\"bibtex\":\"@article{hasegawa_development_2023,\\n\\ttitle = {Development of an extender solution for short-term sperm storage to promote seed production in alfonsino \\\\textit{{Beryx} splendens}},\\n\\tissn = {0021-5392, 1349-998X},\\n\\turl = {https://www.jstage.jst.go.jp/article/suisan/advpub/0/advpub_22-00062/_article/-char/ja/},\\n\\tdoi = {10.2331/suisan.22-00062},\\n\\tlanguage = {en},\\n\\turldate = {2023-05-10},\\n\\tjournal = {NIPPON SUISAN GAKKAISHI},\\n\\tauthor = {Hasegawa, Masatoshi and Inaba, Kazuo and Nagakura, Yasuhiro and Noda, Hiroyuki and Kawai, Noriaki},\\n\\tyear = {2023},\\n\\tpages = {22--00062},\\n}\\n\\n\",\"author_short\":[\"Hasegawa, M.\",\"Inaba, K.\",\"Nagakura, Y.\",\"Noda, H.\",\"Kawai, N.\"],\"key\":\"hasegawa_development_2023\",\"id\":\"hasegawa_development_2023\",\"bibbaseid\":\"hasegawa-inaba-nagakura-noda-kawai-developmentofanextendersolutionforshorttermspermstoragetopromoteseedproductioninalfonsinoiberyxsplendensi-2023\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.jstage.jst.go.jp/article/suisan/advpub/0/advpub_22-00062/_article/-char/ja/\"},\"metadata\":{\"authorlinks\":{}},\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"title\":\"Distinct regulation of two flagella by calcium during chemotaxis of male gametes in the brown alga <i>Mutimo cylindricus</i> (Cutleriaceae, Tilopteridales)\",\"issn\":\"0022-3646, 1529-8817\",\"url\":\"https://onlinelibrary.wiley.com/doi/10.1111/jpy.13422\",\"doi\":\"10.1111/jpy.13422\",\"abstract\":\"Abstract Brown algal male gametes show chemotaxis to the sex pheromone that is released from female gametes. The chemotactic behavior of the male gametes is controlled by the changes in the beating of two flagella known as the anterior and posterior flagellum. Our previous study using Mutimo cylindricus showed that the sex pheromone induced an increment in both the deflection angle of the anterior flagellum and sustained unilateral bend of the posterior flagellum, but the mechanisms regulating these two flagellar waveforms were not fully revealed. In this study, we analyzed the changes in swimming path and flagellar waveforms with a high‐speed recording system under different calcium conditions. The extracellular Ca 2+ concentration at 10 −3  M caused an increment in the deflection angle of the anterior flagellum only when ionomycin was absent. No sustained unilateral bend of the posterior flagellum was induced either in the absence or presence of ionomycin in extracellular Ca 2+ concentrations below 10 −2  M. Real‐time Ca 2+ imaging revealed that there is a spot near the basal part of anterior flagellum showing higher Ca 2+ than in the other parts of the cell. The intensity of the spot slightly decreased when male gametes were treated with the sex pheromone. These results suggest that Ca 2+ ‐dependent changes in the anterior and posterior flagellum are regulated by distinct mechanisms and that the increase in the anterior flagellar deflection angle and sustained unilateral bend of the posterior flagellum may not be primarily induced by the Ca 2+ concentration.\",\"language\":\"en\",\"urldate\":\"2024-04-03\",\"journal\":\"Journal of Phycology\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Kinoshita‐Terauchi\"],\"firstnames\":[\"Nana\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shiba\"],\"firstnames\":[\"Kogiku\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Umezawa\"],\"firstnames\":[\"Taiki\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Inaba\"],\"firstnames\":[\"Kazuo\"],\"suffixes\":[]}],\"month\":\"December\",\"year\":\"2023\",\"pages\":\"jpy.13422\",\"bibtex\":\"@article{kinoshitaterauchi_distinct_2023,\\n\\ttitle = {Distinct regulation of two flagella by calcium during chemotaxis of male gametes in the brown alga \\\\textit{{Mutimo} cylindricus} ({Cutleriaceae}, {Tilopteridales})},\\n\\tissn = {0022-3646, 1529-8817},\\n\\turl = {https://onlinelibrary.wiley.com/doi/10.1111/jpy.13422},\\n\\tdoi = {10.1111/jpy.13422},\\n\\tabstract = {Abstract\\n            \\n              Brown algal male gametes show chemotaxis to the sex pheromone that is released from female gametes. The chemotactic behavior of the male gametes is controlled by the changes in the beating of two flagella known as the anterior and posterior flagellum. Our previous study using\\n              Mutimo cylindricus\\n              showed that the sex pheromone induced an increment in both the deflection angle of the anterior flagellum and sustained unilateral bend of the posterior flagellum, but the mechanisms regulating these two flagellar waveforms were not fully revealed. In this study, we analyzed the changes in swimming path and flagellar waveforms with a high‐speed recording system under different calcium conditions. The extracellular Ca\\n              2+\\n              concentration at 10\\n              −3\\n               M caused an increment in the deflection angle of the anterior flagellum only when ionomycin was absent. No sustained unilateral bend of the posterior flagellum was induced either in the absence or presence of ionomycin in extracellular Ca\\n              2+\\n              concentrations below 10\\n              −2\\n               M. Real‐time Ca\\n              2+\\n              imaging revealed that there is a spot near the basal part of anterior flagellum showing higher Ca\\n              2+\\n              than in the other parts of the cell. The intensity of the spot slightly decreased when male gametes were treated with the sex pheromone. These results suggest that Ca\\n              2+\\n              ‐dependent changes in the anterior and posterior flagellum are regulated by distinct mechanisms and that the increase in the anterior flagellar deflection angle and sustained unilateral bend of the posterior flagellum may not be primarily induced by the Ca\\n              2+\\n              concentration.},\\n\\tlanguage = {en},\\n\\turldate = {2024-04-03},\\n\\tjournal = {Journal of Phycology},\\n\\tauthor = {Kinoshita‐Terauchi, Nana and Shiba, Kogiku and Umezawa, Taiki and Inaba, Kazuo},\\n\\tmonth = dec,\\n\\tyear = {2023},\\n\\tpages = {jpy.13422},\\n}\\n\\n\",\"author_short\":[\"Kinoshita‐Terauchi, N.\",\"Shiba, K.\",\"Umezawa, T.\",\"Inaba, K.\"],\"key\":\"kinoshitaterauchi_distinct_2023\",\"id\":\"kinoshitaterauchi_distinct_2023\",\"bibbaseid\":\"kinoshitaterauchi-shiba-umezawa-inaba-distinctregulationoftwoflagellabycalciumduringchemotaxisofmalegametesinthebrownalgaimutimocylindricusicutleriaceaetilopteridales-2023\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://onlinelibrary.wiley.com/doi/10.1111/jpy.13422\"},\"metadata\":{\"authorlinks\":{}},\"downloads\":1,\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"title\":\"Positive selection on ADAM10 builds species recognition in the synchronous spawning coral <i>Acropora</i>\",\"volume\":\"11\",\"issn\":\"2296-634X\",\"url\":\"https://www.frontiersin.org/articles/10.3389/fcell.2023.1171495/full\",\"doi\":\"10.3389/fcell.2023.1171495\",\"abstract\":\"The reef-building coral Acropora is a broadcast spawning hermaphrodite including more than 110 species in the Indo-Pacific. In addition, many sympatric species show synchronous spawning. The released gametes need to mate with conspecifics in the mixture of the gametes of many species for their species boundaries. However, the mechanism underlying the species recognition of conspecifics at fertilization remains unknown. We hypothesized that rapid molecular evolution (positive selection) in genes encoding gamete-composing proteins generates polymorphic regions that recognize conspecifics in the mixture of gametes from many species. We identified gamete proteins of Acropora digitifera using mass spectrometry and screened the genes that support branch site models that set the “foreground” branches showing strict fertilization specificity. ADAM10, ADAM17, Integrin α9, and Tetraspanin4 supported branch-site model and had positively selected site(s) that produced polymorphic regions. Therefore, we prepared antibodies against the proteins of A. digitifera that contained positively selected site(s) to analyze their functions in fertilization. The ADAM10 antibody reacted only with egg proteins of A. digitifera , and immunohistochemistry showed ADAM10 localized around the egg surface. Moreover, the ADAM10 antibody inhibited only A. digitifera fertilization but not the relative synchronous spawning species A. papillare . This study indicates that ADAM10 has evolved to gain fertilization specificity during speciation and contributes to species boundaries in this multi-species, synchronous-spawning, and species-rich genus.\",\"urldate\":\"2023-05-10\",\"journal\":\"Frontiers in Cell and Developmental Biology\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Morita\"],\"firstnames\":[\"Masaya\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Kitanobo\"],\"firstnames\":[\"Seiya\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Ohki\"],\"firstnames\":[\"Shun\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shiba\"],\"firstnames\":[\"Kogiku\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Inaba\"],\"firstnames\":[\"Kazuo\"],\"suffixes\":[]}],\"month\":\"April\",\"year\":\"2023\",\"pages\":\"1171495\",\"bibtex\":\"@article{morita_positive_2023,\\n\\ttitle = {Positive selection on {ADAM10} builds species recognition in the synchronous spawning coral \\\\textit{{Acropora}}},\\n\\tvolume = {11},\\n\\tissn = {2296-634X},\\n\\turl = {https://www.frontiersin.org/articles/10.3389/fcell.2023.1171495/full},\\n\\tdoi = {10.3389/fcell.2023.1171495},\\n\\tabstract = {The reef-building coral\\n              Acropora\\n              is a broadcast spawning hermaphrodite including more than 110 species in the Indo-Pacific. In addition, many sympatric species show synchronous spawning. The released gametes need to mate with conspecifics in the mixture of the gametes of many species for their species boundaries. However, the mechanism underlying the species recognition of conspecifics at fertilization remains unknown. We hypothesized that rapid molecular evolution (positive selection) in genes encoding gamete-composing proteins generates polymorphic regions that recognize conspecifics in the mixture of gametes from many species. We identified gamete proteins of\\n              Acropora digitifera\\n              using mass spectrometry and screened the genes that support branch site models that set the “foreground” branches showing strict fertilization specificity. ADAM10, ADAM17, Integrin α9, and Tetraspanin4 supported branch-site model and had positively selected site(s) that produced polymorphic regions. Therefore, we prepared antibodies against the proteins of\\n              A. digitifera\\n              that contained positively selected site(s) to analyze their functions in fertilization. The ADAM10 antibody reacted only with egg proteins of\\n              A. digitifera\\n              , and immunohistochemistry showed ADAM10 localized around the egg surface. Moreover, the ADAM10 antibody inhibited only\\n              A. digitifera\\n              fertilization but not the relative synchronous spawning species\\n              A. papillare\\n              . This study indicates that ADAM10 has evolved to gain fertilization specificity during speciation and contributes to species boundaries in this multi-species, synchronous-spawning, and species-rich genus.},\\n\\turldate = {2023-05-10},\\n\\tjournal = {Frontiers in Cell and Developmental Biology},\\n\\tauthor = {Morita, Masaya and Kitanobo, Seiya and Ohki, Shun and Shiba, Kogiku and Inaba, Kazuo},\\n\\tmonth = apr,\\n\\tyear = {2023},\\n\\tpages = {1171495},\\n}\\n\\n\",\"author_short\":[\"Morita, M.\",\"Kitanobo, S.\",\"Ohki, S.\",\"Shiba, K.\",\"Inaba, K.\"],\"key\":\"morita_positive_2023\",\"id\":\"morita_positive_2023\",\"bibbaseid\":\"morita-kitanobo-ohki-shiba-inaba-positiveselectiononadam10buildsspeciesrecognitioninthesynchronousspawningcoraliacroporai-2023\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.frontiersin.org/articles/10.3389/fcell.2023.1171495/full\"},\"metadata\":{\"authorlinks\":{}},\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"title\":\"Spawning-induced pH increase activates sperm attraction and fertilization abilities in eggs of the Ascidian, <i>Phallusia philippinensis</i> and <i>Ciona intestinalis</i>\",\"volume\":\"24\",\"issn\":\"1422-0067\",\"url\":\"https://www.mdpi.com/1422-0067/24/3/2666\",\"doi\":\"10.3390/ijms24032666\",\"abstract\":\"In Phlebobranchiata ascidians, oocytes and spermatozoa are stored in the oviduct and spermiduct, respectively, until spawning occurs. Gametes in the gonoducts are mature and fertilizable; however, it was found that the gametes of the ascidians Phallusia philippinensis and Ciona intestinalis could not undergo fertilization in the gonoductal fluids. The body fluids of the ascidians, especially in the gonoducts, were much more acidic (pH 5.5–6.8) than seawater (pH 8.2), and the fertilization rate was low under such acidic conditions. Hence, we examined the effect of pH on gametes. Pre-incubation of gonoductal eggs at pH 8.2 prior to insemination increased fertilization rates, even when insemination was performed under low pH conditions. Furthermore, an increase in ambient pH induced an increase in the intracellular pH of the eggs. It was also found that an increase in ambient pH triggered the release of sperm attractants from the egg and is therefore necessary for sperm chemotaxis. Hence, acidic conditions in the gonoductal fluids keep the gametes, especially eggs, infertile, and the release of eggs into seawater upon spawning induces an increase in ambient pH, which enables egg fertilization.\",\"language\":\"en\",\"number\":\"3\",\"urldate\":\"2023-05-10\",\"journal\":\"International Journal of Molecular Sciences\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Sensui\"],\"firstnames\":[\"Noburu\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Itoh\"],\"firstnames\":[\"Yosinori\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Okura\"],\"firstnames\":[\"Nobuhiko\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Shiba\"],\"firstnames\":[\"Kogiku\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Baba\"],\"firstnames\":[\"Shoji\",\"A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Inaba\"],\"firstnames\":[\"Kazuo\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Yoshida\"],\"firstnames\":[\"Manabu\"],\"suffixes\":[]}],\"month\":\"January\",\"year\":\"2023\",\"pages\":\"2666\",\"bibtex\":\"@article{sensui_spawning-induced_2023,\\n\\ttitle = {Spawning-induced {pH} increase activates sperm attraction and fertilization abilities in eggs of the {Ascidian}, \\\\textit{{Phallusia} philippinensis} and \\\\textit{{Ciona} intestinalis}},\\n\\tvolume = {24},\\n\\tissn = {1422-0067},\\n\\turl = {https://www.mdpi.com/1422-0067/24/3/2666},\\n\\tdoi = {10.3390/ijms24032666},\\n\\tabstract = {In Phlebobranchiata ascidians, oocytes and spermatozoa are stored in the oviduct and spermiduct, respectively, until spawning occurs. Gametes in the gonoducts are mature and fertilizable; however, it was found that the gametes of the ascidians Phallusia philippinensis and Ciona intestinalis could not undergo fertilization in the gonoductal fluids. The body fluids of the ascidians, especially in the gonoducts, were much more acidic (pH 5.5–6.8) than seawater (pH 8.2), and the fertilization rate was low under such acidic conditions. Hence, we examined the effect of pH on gametes. Pre-incubation of gonoductal eggs at pH 8.2 prior to insemination increased fertilization rates, even when insemination was performed under low pH conditions. Furthermore, an increase in ambient pH induced an increase in the intracellular pH of the eggs. It was also found that an increase in ambient pH triggered the release of sperm attractants from the egg and is therefore necessary for sperm chemotaxis. Hence, acidic conditions in the gonoductal fluids keep the gametes, especially eggs, infertile, and the release of eggs into seawater upon spawning induces an increase in ambient pH, which enables egg fertilization.},\\n\\tlanguage = {en},\\n\\tnumber = {3},\\n\\turldate = {2023-05-10},\\n\\tjournal = {International Journal of Molecular Sciences},\\n\\tauthor = {Sensui, Noburu and Itoh, Yosinori and Okura, Nobuhiko and Shiba, Kogiku and Baba, Shoji A. and Inaba, Kazuo and Yoshida, Manabu},\\n\\tmonth = jan,\\n\\tyear = {2023},\\n\\tpages = {2666},\\n}\\n\\n\",\"author_short\":[\"Sensui, N.\",\"Itoh, Y.\",\"Okura, N.\",\"Shiba, K.\",\"Baba, S. A.\",\"Inaba, K.\",\"Yoshida, M.\"],\"key\":\"sensui_spawning-induced_2023\",\"id\":\"sensui_spawning-induced_2023\",\"bibbaseid\":\"sensui-itoh-okura-shiba-baba-inaba-yoshida-spawninginducedphincreaseactivatesspermattractionandfertilizationabilitiesineggsoftheascidianiphallusiaphilippinensisiandicionaintestinalisi-2023\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.mdpi.com/1422-0067/24/3/2666\"},\"metadata\":{\"authorlinks\":{}},\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"title\":\"Regulatory mechanisms for sperm chemotaxis and flagellar motility\",\"volume\":\"61\",\"issn\":\"1526-954X, 1526-968X\",\"url\":\"https://onlinelibrary.wiley.com/doi/10.1002/dvg.23549\",\"doi\":\"10.1002/dvg.23549\",\"language\":\"en\",\"number\":\"6\",\"urldate\":\"2024-04-03\",\"journal\":\"genesis\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Shiba\"],\"firstnames\":[\"Kogiku\"],\"suffixes\":[]}],\"month\":\"November\",\"year\":\"2023\",\"pages\":\"e23549\",\"bibtex\":\"@article{shiba_regulatory_2023,\\n\\ttitle = {Regulatory mechanisms for sperm chemotaxis and flagellar motility},\\n\\tvolume = {61},\\n\\tissn = {1526-954X, 1526-968X},\\n\\turl = {https://onlinelibrary.wiley.com/doi/10.1002/dvg.23549},\\n\\tdoi = {10.1002/dvg.23549},\\n\\tlanguage = {en},\\n\\tnumber = {6},\\n\\turldate = {2024-04-03},\\n\\tjournal = {genesis},\\n\\tauthor = {Shiba, Kogiku},\\n\\tmonth = nov,\\n\\tyear = {2023},\\n\\tpages = {e23549},\\n}\\n\\n\",\"author_short\":[\"Shiba, K.\"],\"key\":\"shiba_regulatory_2023\",\"id\":\"shiba_regulatory_2023\",\"bibbaseid\":\"shiba-regulatorymechanismsforspermchemotaxisandflagellarmotility-2023\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://onlinelibrary.wiley.com/doi/10.1002/dvg.23549\"},\"metadata\":{\"authorlinks\":{}},\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"title\":\"Calaxin is required for asymmetric bend initiation and propagation in sperm flagella\",\"volume\":\"11\",\"issn\":\"2296-634X\",\"url\":\"https://www.frontiersin.org/articles/10.3389/fcell.2023.1136404/full\",\"doi\":\"10.3389/fcell.2023.1136404\",\"abstract\":\"Regulation of waveform asymmetry in flagella is critical for changes in direction when sperm are swimming, as seen during the chemotaxis of sperm towards eggs. Ca 2+ is an important regulator of asymmetry in flagellar waveforms. A calcium sensor protein, calaxin, is associated with the outer arm dynein and plays a key role in the regulation of flagellar motility in a Ca 2+ -dependent manner. However, the underlying mechanism of regulating asymmetric waves by means of Ca 2+ and calaxin remains unclear. To clarify the calaxin-dependent mechanism for generating Ca 2+ -dependent asymmetric flagellar waveforms, we analyzed the initial step of flagellar bend formation and propagation in the sperm of the ascidian Ciona intestinalis . Our experiment used demembranated sperm cells, which were then reactivated by UV flash photolysis of caged ATP under both high and low Ca 2+ concentrations. Here, we show that initial bends in the flagella are formed at the base of the sperm and propagate towards the tip during waveform generation. However, the direction of the initial bend differed between asymmetric and symmetric waves. When a calaxin inhibitor (repaglinide) was applied, it resulted in the failure of asymmetric wave formation and propagation. This was because repaglinide had no effect on initial bend formation, but it significantly inhibited the generation of the subsequent bend in the reverse direction. Switching of dynein sliding activity by mechanical feedback is crucial for flagellar oscillation. Our results suggest that the Ca 2+ /calaxin mechanism plays an important role in the switching of dynein activity from microtubule sliding in the principal bend into the suppressed sliding in the reverse bend, thereby allowing the sperm to successfully change direction.\",\"urldate\":\"2023-05-10\",\"journal\":\"Frontiers in Cell and Developmental Biology\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Shiba\"],\"firstnames\":[\"Kogiku\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Baba\"],\"firstnames\":[\"Shoji\",\"A.\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Fujiwara\"],\"firstnames\":[\"Eiji\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Inaba\"],\"firstnames\":[\"Kazuo\"],\"suffixes\":[]}],\"month\":\"March\",\"year\":\"2023\",\"pages\":\"1136404\",\"bibtex\":\"@article{shiba_calaxin_2023,\\n\\ttitle = {Calaxin is required for asymmetric bend initiation and propagation in sperm flagella},\\n\\tvolume = {11},\\n\\tissn = {2296-634X},\\n\\turl = {https://www.frontiersin.org/articles/10.3389/fcell.2023.1136404/full},\\n\\tdoi = {10.3389/fcell.2023.1136404},\\n\\tabstract = {Regulation of waveform asymmetry in flagella is critical for changes in direction when sperm are swimming, as seen during the chemotaxis of sperm towards eggs. Ca\\n              2+\\n              is an important regulator of asymmetry in flagellar waveforms. A calcium sensor protein, calaxin, is associated with the outer arm dynein and plays a key role in the regulation of flagellar motility in a Ca\\n              2+\\n              -dependent manner. However, the underlying mechanism of regulating asymmetric waves by means of Ca\\n              2+\\n              and calaxin remains unclear. To clarify the calaxin-dependent mechanism for generating Ca\\n              2+\\n              -dependent asymmetric flagellar waveforms, we analyzed the initial step of flagellar bend formation and propagation in the sperm of the ascidian\\n              Ciona intestinalis\\n              . Our experiment used demembranated sperm cells, which were then reactivated by UV flash photolysis of caged ATP under both high and low Ca\\n              2+\\n              concentrations. Here, we show that initial bends in the flagella are formed at the base of the sperm and propagate towards the tip during waveform generation. However, the direction of the initial bend differed between asymmetric and symmetric waves. When a calaxin inhibitor (repaglinide) was applied, it resulted in the failure of asymmetric wave formation and propagation. This was because repaglinide had no effect on initial bend formation, but it significantly inhibited the generation of the subsequent bend in the reverse direction. Switching of dynein sliding activity by mechanical feedback is crucial for flagellar oscillation. Our results suggest that the Ca\\n              2+\\n              /calaxin mechanism plays an important role in the switching of dynein activity from microtubule sliding in the principal bend into the suppressed sliding in the reverse bend, thereby allowing the sperm to successfully change direction.},\\n\\turldate = {2023-05-10},\\n\\tjournal = {Frontiers in Cell and Developmental Biology},\\n\\tauthor = {Shiba, Kogiku and Baba, Shoji A. and Fujiwara, Eiji and Inaba, Kazuo},\\n\\tmonth = mar,\\n\\tyear = {2023},\\n\\tpages = {1136404},\\n}\\n\\n\",\"author_short\":[\"Shiba, K.\",\"Baba, S. A.\",\"Fujiwara, E.\",\"Inaba, K.\"],\"key\":\"shiba_calaxin_2023\",\"id\":\"shiba_calaxin_2023\",\"bibbaseid\":\"shiba-baba-fujiwara-inaba-calaxinisrequiredforasymmetricbendinitiationandpropagationinspermflagella-2023\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.frontiersin.org/articles/10.3389/fcell.2023.1136404/full\"},\"metadata\":{\"authorlinks\":{}},\"html\":\"\"},{\"bibtype\":\"article\",\"type\":\"article\",\"title\":\"The Role of Soluble Adenylyl Cyclase in the Regulation of Flagellar Motility in Ascidian Sperm\",\"volume\":\"13\",\"copyright\":\"https://creativecommons.org/licenses/by/4.0/\",\"issn\":\"2218-273X\",\"url\":\"https://www.mdpi.com/2218-273X/13/11/1594\",\"doi\":\"10.3390/biom13111594\",\"abstract\":\"Flagellar motility in sperm is activated and regulated by factors related to the eggs at fertilization. In the ascidian Ciona intestinalis, a sulfated steroid called the SAAF (sperm activating and attracting factor) induces both sperm motility activation and chemotaxis. Cyclic AMP (cAMP) is one of the most important intracellular factors in the sperm signaling pathway. Adenylyl cyclase (AC) is the key enzyme that synthesizes cAMP at the onset of the signaling pathway in all cellular functions. We previously reported that both transmembrane AC (tmAC) and soluble AC (sAC) play important roles in sperm motility in Ciona. The tmAC plays a major role in the SAAF-induced activation of sperm motility. On the other hand, sAC is involved in the regulation of flagellar beat frequency and the Ca2+-dependent chemotactic movement of sperm. In this study, we focused on the role of sAC in the regulation of flagellar motility in Ciona sperm chemotaxis. The immunochemical analysis revealed that several isoforms of sAC protein were expressed in Ciona sperm, as reported in mammals and sea urchins. We demonstrated that sAC inhibition caused strong and transient asymmetrization during the chemotactic turn, and then sperm failed to turn toward the SAAF. In addition, real-time Ca2+ imaging in sperm flagella revealed that sAC inhibition induced an excessive and prolonged Ca2+ influx to flagella. These results indicate that sAC plays a key role in sperm chemotaxis by regulating the clearance of [Ca2+]i and by modulating Ca2+-dependent flagellar waveform conversion.\",\"language\":\"en\",\"number\":\"11\",\"urldate\":\"2024-04-03\",\"journal\":\"Biomolecules\",\"author\":[{\"propositions\":[],\"lastnames\":[\"Shiba\"],\"firstnames\":[\"Kogiku\"],\"suffixes\":[]},{\"propositions\":[],\"lastnames\":[\"Inaba\"],\"firstnames\":[\"Kazuo\"],\"suffixes\":[]}],\"month\":\"October\",\"year\":\"2023\",\"pages\":\"1594\",\"bibtex\":\"@article{shiba_role_2023,\\n\\ttitle = {The {Role} of {Soluble} {Adenylyl} {Cyclase} in the {Regulation} of {Flagellar} {Motility} in {Ascidian} {Sperm}},\\n\\tvolume = {13},\\n\\tcopyright = {https://creativecommons.org/licenses/by/4.0/},\\n\\tissn = {2218-273X},\\n\\turl = {https://www.mdpi.com/2218-273X/13/11/1594},\\n\\tdoi = {10.3390/biom13111594},\\n\\tabstract = {Flagellar motility in sperm is activated and regulated by factors related to the eggs at fertilization. In the ascidian Ciona intestinalis, a sulfated steroid called the SAAF (sperm activating and attracting factor) induces both sperm motility activation and chemotaxis. Cyclic AMP (cAMP) is one of the most important intracellular factors in the sperm signaling pathway. Adenylyl cyclase (AC) is the key enzyme that synthesizes cAMP at the onset of the signaling pathway in all cellular functions. We previously reported that both transmembrane AC (tmAC) and soluble AC (sAC) play important roles in sperm motility in Ciona. The tmAC plays a major role in the SAAF-induced activation of sperm motility. On the other hand, sAC is involved in the regulation of flagellar beat frequency and the Ca2+-dependent chemotactic movement of sperm. In this study, we focused on the role of sAC in the regulation of flagellar motility in Ciona sperm chemotaxis. The immunochemical analysis revealed that several isoforms of sAC protein were expressed in Ciona sperm, as reported in mammals and sea urchins. We demonstrated that sAC inhibition caused strong and transient asymmetrization during the chemotactic turn, and then sperm failed to turn toward the SAAF. In addition, real-time Ca2+ imaging in sperm flagella revealed that sAC inhibition induced an excessive and prolonged Ca2+ influx to flagella. These results indicate that sAC plays a key role in sperm chemotaxis by regulating the clearance of [Ca2+]i and by modulating Ca2+-dependent flagellar waveform conversion.},\\n\\tlanguage = {en},\\n\\tnumber = {11},\\n\\turldate = {2024-04-03},\\n\\tjournal = {Biomolecules},\\n\\tauthor = {Shiba, Kogiku and Inaba, Kazuo},\\n\\tmonth = oct,\\n\\tyear = {2023},\\n\\tpages = {1594},\\n}\\n\\n\",\"author_short\":[\"Shiba, K.\",\"Inaba, K.\"],\"key\":\"shiba_role_2023\",\"id\":\"shiba_role_2023\",\"bibbaseid\":\"shiba-inaba-theroleofsolubleadenylylcyclaseintheregulationofflagellarmotilityinascidiansperm-2023\",\"role\":\"author\",\"urls\":{\"Paper\":\"https://www.mdpi.com/2218-273X/13/11/1594\"},\"metadata\":{\"authorlinks\":{}},\"html\":\"\"}],\n      metadata: {\"authorlinks\":{}},\n      ownerID: undefined\n    };\n  </script>\n\n  <script type=\"text/javascript\">\n  var site$;\n  if (typeof $ != 'undefined') {\n    console.log('existing jquery: storing and then restoring');\n    site$ = $;\n    $ = null;\n  }\n  </script>\n\n  <script src=\"https://ajax.googleapis.com/ajax/libs/jquery/2.2.4/jquery.min.js\">\n  </script>\n  <script type=\"text/javascript\">\n  if (site$) {\n    console.log('existing jquery: avoiding conflict and restoring');\n    bb$ = $.noConflict(true);\n    $ = site$;\n  } else {\n    bb$ = $;\n  }\n  </script>\n\n  <script src=\"//bibbase.org/js/bibbase.min.js\"\n          type=\"text/javascript\">\n  </script>\n\n  <script type=\"text/javascript\"\n          src=\"https://cdnjs.cloudflare.com/ajax/libs/mathjax/2.7.1/MathJax.js?config=TeX-AMS-MML_HTMLorMML\">\n  </script>\n  <script type=\"text/x-mathjax-config\">\n    MathJax.Hub.Config({tex2jax: {inlineMath: [['$','$'], ['\\\\(','\\\\)']]},\n                        skipTags: [\"body\"],\n                        processClass: \"bibbase_paper\"});\n  </script>\n\n  <style>\n  @media print {\n    .dontprint {\n        display: none !important;\n    }\n\n  .bibbase_group {\n    background-color: #E0E0E0;\n    font-style: italic;\n    font-size: larger;\n    text-shadow: 0px 0px 3px #FFFFFF;\n    border-radius: 4px 4px 4px 4px;\n    -moz-border-radius: 4px 4px 4px 4px;\n    -webkit-border-radius: 4px;\n    padding: 5px 40px 5px;\n    margin-top: 10px;\n    margin-bottom: 5px;\n    cursor: pointer;\n  }\n\n  .bibbase_paper {\n    margin-bottom: 5px;\n  }\n\n  }\n  </style>\n\n  \n  <div style=\"float: right; margin-right: 10px; margin-top: 10px; text-align: right; font-size: 12px\">\n    generated by\n    <a href=\"//bibbase.org\"\n       onclick=\"trackOutboundLink('bibbase', 'logo clicked', window.location.href, '//bibbase.org'); return false;\">\n      <img src=\"//bibbase.org/img/logo.svg\"\n           style=\"vertical-align: middle; margin-left: 3px; height: 16px;\"/\n           alt=\"bibbase.org\"\n           title=\"BibBase – The easiest way to maintain your publications page.\"\n        ></a>\n    \n  </div>\n  \n\n  <div id=\"bibbase_header\" class=\"dontprint\" style=\"display: none\">\n\n    <ul class=\"nav nav-pills\" style=\"margin: 0px;\">\n\n      <li class=\"dropdown\" id=\"groupby_dropdown\">\n      <a class=\"dropdown-toggle\"\n         data-toggle=\"dropdown\"\n         href=\"#\">\n          Group by\n          <b class=\"caret\"></b>\n      </a>\n      <ul class=\"dropdown-menu\">\n        <li class=\"groupby year\"><a onclick=\"groupby('year')\">\n            Year</a>\n        </li>\n        <li class=\"groupby author\"><a onclick=\"groupby('author_short')\">\n            Author</a>\n        </li>\n        <li class=\"groupby type\"><a onclick=\"groupby('type')\">\n            Type</a>\n        </li>\n        <li class=\"groupby keyword\"><a onclick=\"groupby('keyword')\">\n            Keyword</a>\n        </li>\n        <li class=\"groupby downloads\"><a onclick=\"groupby('downloads')\">\n            Downloads</a>\n        </li>\n      </ul>\n      </li>\n\n\n      <li class=\"dropdown\" id=\"menu_dropdown\">\n      <a class=\"dropdown-toggle\"\n         data-toggle=\"dropdown\"\n         href=\"#\" alt=\"controls\">\n          <i class=\"fa fa-reorder\" alt=\"controls\"></i>\n          <b class=\"caret\"></b>\n      </a>\n      <ul class=\"dropdown-menu\">\n        <li>\n          <a onclick=\"toggleAll()\">\n            <i class=\"fa fa-chevron-down fa-fw\"></i> Expand/Collapse All\n          </a>\n        </li>\n        <li>\n          <a download=\"items\" href=\"data:text/bibtex;base64,@article{hasegawa_development_2023,
	title = {Development of an extender solution for short-term sperm storage to promote seed production in alfonsino \textit{{Beryx} splendens}},
	issn = {0021-5392, 1349-998X},
	url = {https://www.jstage.jst.go.jp/article/suisan/advpub/0/advpub_22-00062/_article/-char/ja/},
	doi = {10.2331/suisan.22-00062},
	language = {en},
	urldate = {2023-05-10},
	journal = {NIPPON SUISAN GAKKAISHI},
	author = {Hasegawa, Masatoshi and Inaba, Kazuo and Nagakura, Yasuhiro and Noda, Hiroyuki and Kawai, Noriaki},
	year = {2023},
	pages = {22--00062},
}



@article{kinoshitaterauchi_distinct_2023,
	title = {Distinct regulation of two flagella by calcium during chemotaxis of male gametes in the brown alga \textit{{Mutimo} cylindricus} ({Cutleriaceae}, {Tilopteridales})},
	issn = {0022-3646, 1529-8817},
	url = {https://onlinelibrary.wiley.com/doi/10.1111/jpy.13422},
	doi = {10.1111/jpy.13422},
	abstract = {Abstract
            
              Brown algal male gametes show chemotaxis to the sex pheromone that is released from female gametes. The chemotactic behavior of the male gametes is controlled by the changes in the beating of two flagella known as the anterior and posterior flagellum. Our previous study using
              Mutimo cylindricus
              showed that the sex pheromone induced an increment in both the deflection angle of the anterior flagellum and sustained unilateral bend of the posterior flagellum, but the mechanisms regulating these two flagellar waveforms were not fully revealed. In this study, we analyzed the changes in swimming path and flagellar waveforms with a high‐speed recording system under different calcium conditions. The extracellular Ca
              2+
              concentration at 10
              −3
               M caused an increment in the deflection angle of the anterior flagellum only when ionomycin was absent. No sustained unilateral bend of the posterior flagellum was induced either in the absence or presence of ionomycin in extracellular Ca
              2+
              concentrations below 10
              −2
               M. Real‐time Ca
              2+
              imaging revealed that there is a spot near the basal part of anterior flagellum showing higher Ca
              2+
              than in the other parts of the cell. The intensity of the spot slightly decreased when male gametes were treated with the sex pheromone. These results suggest that Ca
              2+
              ‐dependent changes in the anterior and posterior flagellum are regulated by distinct mechanisms and that the increase in the anterior flagellar deflection angle and sustained unilateral bend of the posterior flagellum may not be primarily induced by the Ca
              2+
              concentration.},
	language = {en},
	urldate = {2024-04-03},
	journal = {Journal of Phycology},
	author = {Kinoshita‐Terauchi, Nana and Shiba, Kogiku and Umezawa, Taiki and Inaba, Kazuo},
	month = dec,
	year = {2023},
	pages = {jpy.13422},
}



@article{morita_positive_2023,
	title = {Positive selection on {ADAM10} builds species recognition in the synchronous spawning coral \textit{{Acropora}}},
	volume = {11},
	issn = {2296-634X},
	url = {https://www.frontiersin.org/articles/10.3389/fcell.2023.1171495/full},
	doi = {10.3389/fcell.2023.1171495},
	abstract = {The reef-building coral
              Acropora
              is a broadcast spawning hermaphrodite including more than 110 species in the Indo-Pacific. In addition, many sympatric species show synchronous spawning. The released gametes need to mate with conspecifics in the mixture of the gametes of many species for their species boundaries. However, the mechanism underlying the species recognition of conspecifics at fertilization remains unknown. We hypothesized that rapid molecular evolution (positive selection) in genes encoding gamete-composing proteins generates polymorphic regions that recognize conspecifics in the mixture of gametes from many species. We identified gamete proteins of
              Acropora digitifera
              using mass spectrometry and screened the genes that support branch site models that set the “foreground” branches showing strict fertilization specificity. ADAM10, ADAM17, Integrin α9, and Tetraspanin4 supported branch-site model and had positively selected site(s) that produced polymorphic regions. Therefore, we prepared antibodies against the proteins of
              A. digitifera
              that contained positively selected site(s) to analyze their functions in fertilization. The ADAM10 antibody reacted only with egg proteins of
              A. digitifera
              , and immunohistochemistry showed ADAM10 localized around the egg surface. Moreover, the ADAM10 antibody inhibited only
              A. digitifera
              fertilization but not the relative synchronous spawning species
              A. papillare
              . This study indicates that ADAM10 has evolved to gain fertilization specificity during speciation and contributes to species boundaries in this multi-species, synchronous-spawning, and species-rich genus.},
	urldate = {2023-05-10},
	journal = {Frontiers in Cell and Developmental Biology},
	author = {Morita, Masaya and Kitanobo, Seiya and Ohki, Shun and Shiba, Kogiku and Inaba, Kazuo},
	month = apr,
	year = {2023},
	pages = {1171495},
}



@article{sensui_spawning-induced_2023,
	title = {Spawning-induced {pH} increase activates sperm attraction and fertilization abilities in eggs of the {Ascidian}, \textit{{Phallusia} philippinensis} and \textit{{Ciona} intestinalis}},
	volume = {24},
	issn = {1422-0067},
	url = {https://www.mdpi.com/1422-0067/24/3/2666},
	doi = {10.3390/ijms24032666},
	abstract = {In Phlebobranchiata ascidians, oocytes and spermatozoa are stored in the oviduct and spermiduct, respectively, until spawning occurs. Gametes in the gonoducts are mature and fertilizable; however, it was found that the gametes of the ascidians Phallusia philippinensis and Ciona intestinalis could not undergo fertilization in the gonoductal fluids. The body fluids of the ascidians, especially in the gonoducts, were much more acidic (pH 5.5–6.8) than seawater (pH 8.2), and the fertilization rate was low under such acidic conditions. Hence, we examined the effect of pH on gametes. Pre-incubation of gonoductal eggs at pH 8.2 prior to insemination increased fertilization rates, even when insemination was performed under low pH conditions. Furthermore, an increase in ambient pH induced an increase in the intracellular pH of the eggs. It was also found that an increase in ambient pH triggered the release of sperm attractants from the egg and is therefore necessary for sperm chemotaxis. Hence, acidic conditions in the gonoductal fluids keep the gametes, especially eggs, infertile, and the release of eggs into seawater upon spawning induces an increase in ambient pH, which enables egg fertilization.},
	language = {en},
	number = {3},
	urldate = {2023-05-10},
	journal = {International Journal of Molecular Sciences},
	author = {Sensui, Noburu and Itoh, Yosinori and Okura, Nobuhiko and Shiba, Kogiku and Baba, Shoji A. and Inaba, Kazuo and Yoshida, Manabu},
	month = jan,
	year = {2023},
	pages = {2666},
}



@article{shiba_regulatory_2023,
	title = {Regulatory mechanisms for sperm chemotaxis and flagellar motility},
	volume = {61},
	issn = {1526-954X, 1526-968X},
	url = {https://onlinelibrary.wiley.com/doi/10.1002/dvg.23549},
	doi = {10.1002/dvg.23549},
	language = {en},
	number = {6},
	urldate = {2024-04-03},
	journal = {genesis},
	author = {Shiba, Kogiku},
	month = nov,
	year = {2023},
	pages = {e23549},
}



@article{shiba_calaxin_2023,
	title = {Calaxin is required for asymmetric bend initiation and propagation in sperm flagella},
	volume = {11},
	issn = {2296-634X},
	url = {https://www.frontiersin.org/articles/10.3389/fcell.2023.1136404/full},
	doi = {10.3389/fcell.2023.1136404},
	abstract = {Regulation of waveform asymmetry in flagella is critical for changes in direction when sperm are swimming, as seen during the chemotaxis of sperm towards eggs. Ca
              2+
              is an important regulator of asymmetry in flagellar waveforms. A calcium sensor protein, calaxin, is associated with the outer arm dynein and plays a key role in the regulation of flagellar motility in a Ca
              2+
              -dependent manner. However, the underlying mechanism of regulating asymmetric waves by means of Ca
              2+
              and calaxin remains unclear. To clarify the calaxin-dependent mechanism for generating Ca
              2+
              -dependent asymmetric flagellar waveforms, we analyzed the initial step of flagellar bend formation and propagation in the sperm of the ascidian
              Ciona intestinalis
              . Our experiment used demembranated sperm cells, which were then reactivated by UV flash photolysis of caged ATP under both high and low Ca
              2+
              concentrations. Here, we show that initial bends in the flagella are formed at the base of the sperm and propagate towards the tip during waveform generation. However, the direction of the initial bend differed between asymmetric and symmetric waves. When a calaxin inhibitor (repaglinide) was applied, it resulted in the failure of asymmetric wave formation and propagation. This was because repaglinide had no effect on initial bend formation, but it significantly inhibited the generation of the subsequent bend in the reverse direction. Switching of dynein sliding activity by mechanical feedback is crucial for flagellar oscillation. Our results suggest that the Ca
              2+
              /calaxin mechanism plays an important role in the switching of dynein activity from microtubule sliding in the principal bend into the suppressed sliding in the reverse bend, thereby allowing the sperm to successfully change direction.},
	urldate = {2023-05-10},
	journal = {Frontiers in Cell and Developmental Biology},
	author = {Shiba, Kogiku and Baba, Shoji A. and Fujiwara, Eiji and Inaba, Kazuo},
	month = mar,
	year = {2023},
	pages = {1136404},
}



@article{shiba_role_2023,
	title = {The {Role} of {Soluble} {Adenylyl} {Cyclase} in the {Regulation} of {Flagellar} {Motility} in {Ascidian} {Sperm}},
	volume = {13},
	copyright = {https://creativecommons.org/licenses/by/4.0/},
	issn = {2218-273X},
	url = {https://www.mdpi.com/2218-273X/13/11/1594},
	doi = {10.3390/biom13111594},
	abstract = {Flagellar motility in sperm is activated and regulated by factors related to the eggs at fertilization. In the ascidian Ciona intestinalis, a sulfated steroid called the SAAF (sperm activating and attracting factor) induces both sperm motility activation and chemotaxis. Cyclic AMP (cAMP) is one of the most important intracellular factors in the sperm signaling pathway. Adenylyl cyclase (AC) is the key enzyme that synthesizes cAMP at the onset of the signaling pathway in all cellular functions. We previously reported that both transmembrane AC (tmAC) and soluble AC (sAC) play important roles in sperm motility in Ciona. The tmAC plays a major role in the SAAF-induced activation of sperm motility. On the other hand, sAC is involved in the regulation of flagellar beat frequency and the Ca2+-dependent chemotactic movement of sperm. In this study, we focused on the role of sAC in the regulation of flagellar motility in Ciona sperm chemotaxis. The immunochemical analysis revealed that several isoforms of sAC protein were expressed in Ciona sperm, as reported in mammals and sea urchins. We demonstrated that sAC inhibition caused strong and transient asymmetrization during the chemotactic turn, and then sperm failed to turn toward the SAAF. In addition, real-time Ca2+ imaging in sperm flagella revealed that sAC inhibition induced an excessive and prolonged Ca2+ influx to flagella. These results indicate that sAC plays a key role in sperm chemotaxis by regulating the clearance of [Ca2+]i and by modulating Ca2+-dependent flagellar waveform conversion.},
	language = {en},
	number = {11},
	urldate = {2024-04-03},
	journal = {Biomolecules},
	author = {Shiba, Kogiku and Inaba, Kazuo},
	month = oct,
	year = {2023},
	pages = {1594},
}

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[link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.2331/suisan.22-00062\"\n     onclick=\"log_download('hasegawa-inaba-nagakura-noda-kawai-developmentofanextendersolutionforshorttermspermstoragetopromoteseedproductioninalfonsinoiberyxsplendensi-2023', 'http://doi.org/10.2331/suisan.22-00062', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/hasegawa-inaba-nagakura-noda-kawai-developmentofanextendersolutionforshorttermspermstoragetopromoteseedproductioninalfonsinoiberyxsplendensi-2023\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('hasegawa-inaba-nagakura-noda-kawai-developmentofanextendersolutionforshorttermspermstoragetopromoteseedproductioninalfonsinoiberyxsplendensi-2023')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@article{hasegawa_development_2023,\n\ttitle = {Development of an extender solution for short-term sperm storage to promote seed production in alfonsino \\textit{{Beryx} splendens}},\n\tissn = {0021-5392, 1349-998X},\n\turl = {https://www.jstage.jst.go.jp/article/suisan/advpub/0/advpub_22-00062/_article/-char/ja/},\n\tdoi = {10.2331/suisan.22-00062},\n\tlanguage = {en},\n\turldate = {2023-05-10},\n\tjournal = {NIPPON SUISAN GAKKAISHI},\n\tauthor = {Hasegawa, Masatoshi and Inaba, Kazuo and Nagakura, Yasuhiro and Noda, Hiroyuki and Kawai, Noriaki},\n\tyear = {2023},\n\tpages = {22--00062},\n}\n\n</pre>\n</div>\n\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"kinoshitaterauchi-shiba-umezawa-inaba-distinctregulationoftwoflagellabycalciumduringchemotaxisofmalegametesinthebrownalgaimutimocylindricusicutleriaceaetilopteridales-2023\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_author\">\n  Kinoshita‐Terauchi, N., Shiba, K., Umezawa, T.,  &amp; Inaba, K.\n</span>\n\n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://onlinelibrary.wiley.com/doi/10.1111/jpy.13422\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'kinoshitaterauchi-shiba-umezawa-inaba-distinctregulationoftwoflagellabycalciumduringchemotaxisofmalegametesinthebrownalgaimutimocylindricusicutleriaceaetilopteridales-2023', 'https://onlinelibrary.wiley.com/doi/10.1111/jpy.13422', event);\">\n    Distinct regulation of two flagella by calcium during chemotaxis of male gametes in the brown alga <i>Mutimo cylindricus</i> (Cutleriaceae, Tilopteridales).\n  </a>\n  \n  \n  \n</span>\n\n  \n\n</span>\n\n  <i>Journal of Phycology</i>,jpy.13422. December 2023.\n  <span class=\"note\"></span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://onlinelibrary.wiley.com/doi/10.1111/jpy.13422\"\n     onclick=\"log_download('kinoshitaterauchi-shiba-umezawa-inaba-distinctregulationoftwoflagellabycalciumduringchemotaxisofmalegametesinthebrownalgaimutimocylindricusicutleriaceaetilopteridales-2023', 'https://onlinelibrary.wiley.com/doi/10.1111/jpy.13422', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Distinct regulation of two flagella by calcium during chemotaxis of male gametes in the brown alga &lt;i&gt;Mutimo cylindricus&lt;/i&gt; (Cutleriaceae, Tilopteridales) [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1111/jpy.13422\"\n     onclick=\"log_download('kinoshitaterauchi-shiba-umezawa-inaba-distinctregulationoftwoflagellabycalciumduringchemotaxisofmalegametesinthebrownalgaimutimocylindricusicutleriaceaetilopteridales-2023', 'http://doi.org/10.1111/jpy.13422', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/kinoshitaterauchi-shiba-umezawa-inaba-distinctregulationoftwoflagellabycalciumduringchemotaxisofmalegametesinthebrownalgaimutimocylindricusicutleriaceaetilopteridales-2023\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n  &nbsp;\n  <span class=\"bibbase_stats_paper\" style=\"color: #777;\">\n    <span>1 download</span>\n  </span>\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('kinoshitaterauchi-shiba-umezawa-inaba-distinctregulationoftwoflagellabycalciumduringchemotaxisofmalegametesinthebrownalgaimutimocylindricusicutleriaceaetilopteridales-2023')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@article{kinoshitaterauchi_distinct_2023,\n\ttitle = {Distinct regulation of two flagella by calcium during chemotaxis of male gametes in the brown alga \\textit{{Mutimo} cylindricus} ({Cutleriaceae}, {Tilopteridales})},\n\tissn = {0022-3646, 1529-8817},\n\turl = {https://onlinelibrary.wiley.com/doi/10.1111/jpy.13422},\n\tdoi = {10.1111/jpy.13422},\n\tabstract = {Abstract\n            \n              Brown algal male gametes show chemotaxis to the sex pheromone that is released from female gametes. The chemotactic behavior of the male gametes is controlled by the changes in the beating of two flagella known as the anterior and posterior flagellum. Our previous study using\n              Mutimo cylindricus\n              showed that the sex pheromone induced an increment in both the deflection angle of the anterior flagellum and sustained unilateral bend of the posterior flagellum, but the mechanisms regulating these two flagellar waveforms were not fully revealed. In this study, we analyzed the changes in swimming path and flagellar waveforms with a high‐speed recording system under different calcium conditions. The extracellular Ca\n              2+\n              concentration at 10\n              −3\n               M caused an increment in the deflection angle of the anterior flagellum only when ionomycin was absent. No sustained unilateral bend of the posterior flagellum was induced either in the absence or presence of ionomycin in extracellular Ca\n              2+\n              concentrations below 10\n              −2\n               M. Real‐time Ca\n              2+\n              imaging revealed that there is a spot near the basal part of anterior flagellum showing higher Ca\n              2+\n              than in the other parts of the cell. The intensity of the spot slightly decreased when male gametes were treated with the sex pheromone. These results suggest that Ca\n              2+\n              ‐dependent changes in the anterior and posterior flagellum are regulated by distinct mechanisms and that the increase in the anterior flagellar deflection angle and sustained unilateral bend of the posterior flagellum may not be primarily induced by the Ca\n              2+\n              concentration.},\n\tlanguage = {en},\n\turldate = {2024-04-03},\n\tjournal = {Journal of Phycology},\n\tauthor = {Kinoshita‐Terauchi, Nana and Shiba, Kogiku and Umezawa, Taiki and Inaba, Kazuo},\n\tmonth = dec,\n\tyear = {2023},\n\tpages = {jpy.13422},\n}\n\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Abstract Brown algal male gametes show chemotaxis to the sex pheromone that is released from female gametes. The chemotactic behavior of the male gametes is controlled by the changes in the beating of two flagella known as the anterior and posterior flagellum. Our previous study using Mutimo cylindricus showed that the sex pheromone induced an increment in both the deflection angle of the anterior flagellum and sustained unilateral bend of the posterior flagellum, but the mechanisms regulating these two flagellar waveforms were not fully revealed. In this study, we analyzed the changes in swimming path and flagellar waveforms with a high‐speed recording system under different calcium conditions. The extracellular Ca 2+ concentration at 10 −3  M caused an increment in the deflection angle of the anterior flagellum only when ionomycin was absent. No sustained unilateral bend of the posterior flagellum was induced either in the absence or presence of ionomycin in extracellular Ca 2+ concentrations below 10 −2  M. Real‐time Ca 2+ imaging revealed that there is a spot near the basal part of anterior flagellum showing higher Ca 2+ than in the other parts of the cell. The intensity of the spot slightly decreased when male gametes were treated with the sex pheromone. These results suggest that Ca 2+ ‐dependent changes in the anterior and posterior flagellum are regulated by distinct mechanisms and that the increase in the anterior flagellar deflection angle and sustained unilateral bend of the posterior flagellum may not be primarily induced by the Ca 2+ concentration.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"morita-kitanobo-ohki-shiba-inaba-positiveselectiononadam10buildsspeciesrecognitioninthesynchronousspawningcoraliacroporai-2023\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_author\">\n  Morita, M., Kitanobo, S., Ohki, S., Shiba, K.,  &amp; Inaba, K.\n</span>\n\n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.frontiersin.org/articles/10.3389/fcell.2023.1171495/full\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'morita-kitanobo-ohki-shiba-inaba-positiveselectiononadam10buildsspeciesrecognitioninthesynchronousspawningcoraliacroporai-2023', 'https://www.frontiersin.org/articles/10.3389/fcell.2023.1171495/full', event);\">\n    Positive selection on ADAM10 builds species recognition in the synchronous spawning coral <i>Acropora</i>.\n  </a>\n  \n  \n  \n</span>\n\n  \n\n</span>\n\n  <i>Frontiers in Cell and Developmental Biology</i>, 11: 1171495. April 2023.\n  <span class=\"note\"></span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.frontiersin.org/articles/10.3389/fcell.2023.1171495/full\"\n     onclick=\"log_download('morita-kitanobo-ohki-shiba-inaba-positiveselectiononadam10buildsspeciesrecognitioninthesynchronousspawningcoraliacroporai-2023', 'https://www.frontiersin.org/articles/10.3389/fcell.2023.1171495/full', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Positive selection on ADAM10 builds species recognition in the synchronous spawning coral &lt;i&gt;Acropora&lt;/i&gt; [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.3389/fcell.2023.1171495\"\n     onclick=\"log_download('morita-kitanobo-ohki-shiba-inaba-positiveselectiononadam10buildsspeciesrecognitioninthesynchronousspawningcoraliacroporai-2023', 'http://doi.org/10.3389/fcell.2023.1171495', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/morita-kitanobo-ohki-shiba-inaba-positiveselectiononadam10buildsspeciesrecognitioninthesynchronousspawningcoraliacroporai-2023\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('morita-kitanobo-ohki-shiba-inaba-positiveselectiononadam10buildsspeciesrecognitioninthesynchronousspawningcoraliacroporai-2023')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@article{morita_positive_2023,\n\ttitle = {Positive selection on {ADAM10} builds species recognition in the synchronous spawning coral \\textit{{Acropora}}},\n\tvolume = {11},\n\tissn = {2296-634X},\n\turl = {https://www.frontiersin.org/articles/10.3389/fcell.2023.1171495/full},\n\tdoi = {10.3389/fcell.2023.1171495},\n\tabstract = {The reef-building coral\n              Acropora\n              is a broadcast spawning hermaphrodite including more than 110 species in the Indo-Pacific. In addition, many sympatric species show synchronous spawning. The released gametes need to mate with conspecifics in the mixture of the gametes of many species for their species boundaries. However, the mechanism underlying the species recognition of conspecifics at fertilization remains unknown. We hypothesized that rapid molecular evolution (positive selection) in genes encoding gamete-composing proteins generates polymorphic regions that recognize conspecifics in the mixture of gametes from many species. We identified gamete proteins of\n              Acropora digitifera\n              using mass spectrometry and screened the genes that support branch site models that set the “foreground” branches showing strict fertilization specificity. ADAM10, ADAM17, Integrin α9, and Tetraspanin4 supported branch-site model and had positively selected site(s) that produced polymorphic regions. Therefore, we prepared antibodies against the proteins of\n              A. digitifera\n              that contained positively selected site(s) to analyze their functions in fertilization. The ADAM10 antibody reacted only with egg proteins of\n              A. digitifera\n              , and immunohistochemistry showed ADAM10 localized around the egg surface. Moreover, the ADAM10 antibody inhibited only\n              A. digitifera\n              fertilization but not the relative synchronous spawning species\n              A. papillare\n              . This study indicates that ADAM10 has evolved to gain fertilization specificity during speciation and contributes to species boundaries in this multi-species, synchronous-spawning, and species-rich genus.},\n\turldate = {2023-05-10},\n\tjournal = {Frontiers in Cell and Developmental Biology},\n\tauthor = {Morita, Masaya and Kitanobo, Seiya and Ohki, Shun and Shiba, Kogiku and Inaba, Kazuo},\n\tmonth = apr,\n\tyear = {2023},\n\tpages = {1171495},\n}\n\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  The reef-building coral Acropora is a broadcast spawning hermaphrodite including more than 110 species in the Indo-Pacific. In addition, many sympatric species show synchronous spawning. The released gametes need to mate with conspecifics in the mixture of the gametes of many species for their species boundaries. However, the mechanism underlying the species recognition of conspecifics at fertilization remains unknown. We hypothesized that rapid molecular evolution (positive selection) in genes encoding gamete-composing proteins generates polymorphic regions that recognize conspecifics in the mixture of gametes from many species. We identified gamete proteins of Acropora digitifera using mass spectrometry and screened the genes that support branch site models that set the “foreground” branches showing strict fertilization specificity. ADAM10, ADAM17, Integrin α9, and Tetraspanin4 supported branch-site model and had positively selected site(s) that produced polymorphic regions. Therefore, we prepared antibodies against the proteins of A. digitifera that contained positively selected site(s) to analyze their functions in fertilization. The ADAM10 antibody reacted only with egg proteins of A. digitifera , and immunohistochemistry showed ADAM10 localized around the egg surface. Moreover, the ADAM10 antibody inhibited only A. digitifera fertilization but not the relative synchronous spawning species A. papillare . This study indicates that ADAM10 has evolved to gain fertilization specificity during speciation and contributes to species boundaries in this multi-species, synchronous-spawning, and species-rich genus.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"sensui-itoh-okura-shiba-baba-inaba-yoshida-spawninginducedphincreaseactivatesspermattractionandfertilizationabilitiesineggsoftheascidianiphallusiaphilippinensisiandicionaintestinalisi-2023\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_author\">\n  Sensui, N., Itoh, Y., Okura, N., Shiba, K., Baba, S. A., Inaba, K.,  &amp; Yoshida, M.\n</span>\n\n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.mdpi.com/1422-0067/24/3/2666\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'sensui-itoh-okura-shiba-baba-inaba-yoshida-spawninginducedphincreaseactivatesspermattractionandfertilizationabilitiesineggsoftheascidianiphallusiaphilippinensisiandicionaintestinalisi-2023', 'https://www.mdpi.com/1422-0067/24/3/2666', event);\">\n    Spawning-induced pH increase activates sperm attraction and fertilization abilities in eggs of the Ascidian, <i>Phallusia philippinensis</i> and <i>Ciona intestinalis</i>.\n  </a>\n  \n  \n  \n</span>\n\n  \n\n</span>\n\n  <i>International Journal of Molecular Sciences</i>, 24(3): 2666. January 2023.\n  <span class=\"note\"></span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.mdpi.com/1422-0067/24/3/2666\"\n     onclick=\"log_download('sensui-itoh-okura-shiba-baba-inaba-yoshida-spawninginducedphincreaseactivatesspermattractionandfertilizationabilitiesineggsoftheascidianiphallusiaphilippinensisiandicionaintestinalisi-2023', 'https://www.mdpi.com/1422-0067/24/3/2666', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Spawning-induced pH increase activates sperm attraction and fertilization abilities in eggs of the Ascidian, &lt;i&gt;Phallusia philippinensis&lt;/i&gt; and &lt;i&gt;Ciona intestinalis&lt;/i&gt; [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.3390/ijms24032666\"\n     onclick=\"log_download('sensui-itoh-okura-shiba-baba-inaba-yoshida-spawninginducedphincreaseactivatesspermattractionandfertilizationabilitiesineggsoftheascidianiphallusiaphilippinensisiandicionaintestinalisi-2023', 'http://doi.org/10.3390/ijms24032666', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/sensui-itoh-okura-shiba-baba-inaba-yoshida-spawninginducedphincreaseactivatesspermattractionandfertilizationabilitiesineggsoftheascidianiphallusiaphilippinensisiandicionaintestinalisi-2023\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('sensui-itoh-okura-shiba-baba-inaba-yoshida-spawninginducedphincreaseactivatesspermattractionandfertilizationabilitiesineggsoftheascidianiphallusiaphilippinensisiandicionaintestinalisi-2023')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@article{sensui_spawning-induced_2023,\n\ttitle = {Spawning-induced {pH} increase activates sperm attraction and fertilization abilities in eggs of the {Ascidian}, \\textit{{Phallusia} philippinensis} and \\textit{{Ciona} intestinalis}},\n\tvolume = {24},\n\tissn = {1422-0067},\n\turl = {https://www.mdpi.com/1422-0067/24/3/2666},\n\tdoi = {10.3390/ijms24032666},\n\tabstract = {In Phlebobranchiata ascidians, oocytes and spermatozoa are stored in the oviduct and spermiduct, respectively, until spawning occurs. Gametes in the gonoducts are mature and fertilizable; however, it was found that the gametes of the ascidians Phallusia philippinensis and Ciona intestinalis could not undergo fertilization in the gonoductal fluids. The body fluids of the ascidians, especially in the gonoducts, were much more acidic (pH 5.5–6.8) than seawater (pH 8.2), and the fertilization rate was low under such acidic conditions. Hence, we examined the effect of pH on gametes. Pre-incubation of gonoductal eggs at pH 8.2 prior to insemination increased fertilization rates, even when insemination was performed under low pH conditions. Furthermore, an increase in ambient pH induced an increase in the intracellular pH of the eggs. It was also found that an increase in ambient pH triggered the release of sperm attractants from the egg and is therefore necessary for sperm chemotaxis. Hence, acidic conditions in the gonoductal fluids keep the gametes, especially eggs, infertile, and the release of eggs into seawater upon spawning induces an increase in ambient pH, which enables egg fertilization.},\n\tlanguage = {en},\n\tnumber = {3},\n\turldate = {2023-05-10},\n\tjournal = {International Journal of Molecular Sciences},\n\tauthor = {Sensui, Noburu and Itoh, Yosinori and Okura, Nobuhiko and Shiba, Kogiku and Baba, Shoji A. and Inaba, Kazuo and Yoshida, Manabu},\n\tmonth = jan,\n\tyear = {2023},\n\tpages = {2666},\n}\n\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  In Phlebobranchiata ascidians, oocytes and spermatozoa are stored in the oviduct and spermiduct, respectively, until spawning occurs. Gametes in the gonoducts are mature and fertilizable; however, it was found that the gametes of the ascidians Phallusia philippinensis and Ciona intestinalis could not undergo fertilization in the gonoductal fluids. The body fluids of the ascidians, especially in the gonoducts, were much more acidic (pH 5.5–6.8) than seawater (pH 8.2), and the fertilization rate was low under such acidic conditions. Hence, we examined the effect of pH on gametes. Pre-incubation of gonoductal eggs at pH 8.2 prior to insemination increased fertilization rates, even when insemination was performed under low pH conditions. Furthermore, an increase in ambient pH induced an increase in the intracellular pH of the eggs. It was also found that an increase in ambient pH triggered the release of sperm attractants from the egg and is therefore necessary for sperm chemotaxis. Hence, acidic conditions in the gonoductal fluids keep the gametes, especially eggs, infertile, and the release of eggs into seawater upon spawning induces an increase in ambient pH, which enables egg fertilization.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"shiba-regulatorymechanismsforspermchemotaxisandflagellarmotility-2023\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_author\">\n  Shiba, K.\n</span>\n\n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://onlinelibrary.wiley.com/doi/10.1002/dvg.23549\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'shiba-regulatorymechanismsforspermchemotaxisandflagellarmotility-2023', 'https://onlinelibrary.wiley.com/doi/10.1002/dvg.23549', event);\">\n    Regulatory mechanisms for sperm chemotaxis and flagellar motility.\n  </a>\n  \n  \n  \n</span>\n\n  \n\n</span>\n\n  <i>genesis</i>, 61(6): e23549. November 2023.\n  <span class=\"note\"></span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://onlinelibrary.wiley.com/doi/10.1002/dvg.23549\"\n     onclick=\"log_download('shiba-regulatorymechanismsforspermchemotaxisandflagellarmotility-2023', 'https://onlinelibrary.wiley.com/doi/10.1002/dvg.23549', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Regulatory mechanisms for sperm chemotaxis and flagellar motility [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.1002/dvg.23549\"\n     onclick=\"log_download('shiba-regulatorymechanismsforspermchemotaxisandflagellarmotility-2023', 'http://doi.org/10.1002/dvg.23549', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/shiba-regulatorymechanismsforspermchemotaxisandflagellarmotility-2023\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('shiba-regulatorymechanismsforspermchemotaxisandflagellarmotility-2023')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@article{shiba_regulatory_2023,\n\ttitle = {Regulatory mechanisms for sperm chemotaxis and flagellar motility},\n\tvolume = {61},\n\tissn = {1526-954X, 1526-968X},\n\turl = {https://onlinelibrary.wiley.com/doi/10.1002/dvg.23549},\n\tdoi = {10.1002/dvg.23549},\n\tlanguage = {en},\n\tnumber = {6},\n\turldate = {2024-04-03},\n\tjournal = {genesis},\n\tauthor = {Shiba, Kogiku},\n\tmonth = nov,\n\tyear = {2023},\n\tpages = {e23549},\n}\n\n</pre>\n</div>\n\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"shiba-baba-fujiwara-inaba-calaxinisrequiredforasymmetricbendinitiationandpropagationinspermflagella-2023\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_author\">\n  Shiba, K., Baba, S. A., Fujiwara, E.,  &amp; Inaba, K.\n</span>\n\n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.frontiersin.org/articles/10.3389/fcell.2023.1136404/full\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'shiba-baba-fujiwara-inaba-calaxinisrequiredforasymmetricbendinitiationandpropagationinspermflagella-2023', 'https://www.frontiersin.org/articles/10.3389/fcell.2023.1136404/full', event);\">\n    Calaxin is required for asymmetric bend initiation and propagation in sperm flagella.\n  </a>\n  \n  \n  \n</span>\n\n  \n\n</span>\n\n  <i>Frontiers in Cell and Developmental Biology</i>, 11: 1136404. March 2023.\n  <span class=\"note\"></span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.frontiersin.org/articles/10.3389/fcell.2023.1136404/full\"\n     onclick=\"log_download('shiba-baba-fujiwara-inaba-calaxinisrequiredforasymmetricbendinitiationandpropagationinspermflagella-2023', 'https://www.frontiersin.org/articles/10.3389/fcell.2023.1136404/full', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"Calaxin is required for asymmetric bend initiation and propagation in sperm flagella [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.3389/fcell.2023.1136404\"\n     onclick=\"log_download('shiba-baba-fujiwara-inaba-calaxinisrequiredforasymmetricbendinitiationandpropagationinspermflagella-2023', 'http://doi.org/10.3389/fcell.2023.1136404', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/shiba-baba-fujiwara-inaba-calaxinisrequiredforasymmetricbendinitiationandpropagationinspermflagella-2023\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('shiba-baba-fujiwara-inaba-calaxinisrequiredforasymmetricbendinitiationandpropagationinspermflagella-2023')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@article{shiba_calaxin_2023,\n\ttitle = {Calaxin is required for asymmetric bend initiation and propagation in sperm flagella},\n\tvolume = {11},\n\tissn = {2296-634X},\n\turl = {https://www.frontiersin.org/articles/10.3389/fcell.2023.1136404/full},\n\tdoi = {10.3389/fcell.2023.1136404},\n\tabstract = {Regulation of waveform asymmetry in flagella is critical for changes in direction when sperm are swimming, as seen during the chemotaxis of sperm towards eggs. Ca\n              2+\n              is an important regulator of asymmetry in flagellar waveforms. A calcium sensor protein, calaxin, is associated with the outer arm dynein and plays a key role in the regulation of flagellar motility in a Ca\n              2+\n              -dependent manner. However, the underlying mechanism of regulating asymmetric waves by means of Ca\n              2+\n              and calaxin remains unclear. To clarify the calaxin-dependent mechanism for generating Ca\n              2+\n              -dependent asymmetric flagellar waveforms, we analyzed the initial step of flagellar bend formation and propagation in the sperm of the ascidian\n              Ciona intestinalis\n              . Our experiment used demembranated sperm cells, which were then reactivated by UV flash photolysis of caged ATP under both high and low Ca\n              2+\n              concentrations. Here, we show that initial bends in the flagella are formed at the base of the sperm and propagate towards the tip during waveform generation. However, the direction of the initial bend differed between asymmetric and symmetric waves. When a calaxin inhibitor (repaglinide) was applied, it resulted in the failure of asymmetric wave formation and propagation. This was because repaglinide had no effect on initial bend formation, but it significantly inhibited the generation of the subsequent bend in the reverse direction. Switching of dynein sliding activity by mechanical feedback is crucial for flagellar oscillation. Our results suggest that the Ca\n              2+\n              /calaxin mechanism plays an important role in the switching of dynein activity from microtubule sliding in the principal bend into the suppressed sliding in the reverse bend, thereby allowing the sperm to successfully change direction.},\n\turldate = {2023-05-10},\n\tjournal = {Frontiers in Cell and Developmental Biology},\n\tauthor = {Shiba, Kogiku and Baba, Shoji A. and Fujiwara, Eiji and Inaba, Kazuo},\n\tmonth = mar,\n\tyear = {2023},\n\tpages = {1136404},\n}\n\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Regulation of waveform asymmetry in flagella is critical for changes in direction when sperm are swimming, as seen during the chemotaxis of sperm towards eggs. Ca 2+ is an important regulator of asymmetry in flagellar waveforms. A calcium sensor protein, calaxin, is associated with the outer arm dynein and plays a key role in the regulation of flagellar motility in a Ca 2+ -dependent manner. However, the underlying mechanism of regulating asymmetric waves by means of Ca 2+ and calaxin remains unclear. To clarify the calaxin-dependent mechanism for generating Ca 2+ -dependent asymmetric flagellar waveforms, we analyzed the initial step of flagellar bend formation and propagation in the sperm of the ascidian Ciona intestinalis . Our experiment used demembranated sperm cells, which were then reactivated by UV flash photolysis of caged ATP under both high and low Ca 2+ concentrations. Here, we show that initial bends in the flagella are formed at the base of the sperm and propagate towards the tip during waveform generation. However, the direction of the initial bend differed between asymmetric and symmetric waves. When a calaxin inhibitor (repaglinide) was applied, it resulted in the failure of asymmetric wave formation and propagation. This was because repaglinide had no effect on initial bend formation, but it significantly inhibited the generation of the subsequent bend in the reverse direction. Switching of dynein sliding activity by mechanical feedback is crucial for flagellar oscillation. Our results suggest that the Ca 2+ /calaxin mechanism plays an important role in the switching of dynein activity from microtubule sliding in the principal bend into the suppressed sliding in the reverse bend, thereby allowing the sperm to successfully change direction.\n</div>\n\n\n</div>\n\n\n  <div class=\"bibbase_paper\" id=\"shiba-inaba-theroleofsolubleadenylylcyclaseintheregulationofflagellarmotilityinascidiansperm-2023\">\n  <span class=\"bibbase_paper_titleauthoryear\">\n\n  \n  <span class=\"bibbase_paper_author\">\n  Shiba, K.,  &amp; Inaba, K.\n</span>\n\n  <span class=\"bibbase_paper_title\">\n  \n  \n  \n  <a href=\"https://www.mdpi.com/2218-273X/13/11/1594\"\n     onclick=\"return trackOutboundLink('publications', 'click', 'shiba-inaba-theroleofsolubleadenylylcyclaseintheregulationofflagellarmotilityinascidiansperm-2023', 'https://www.mdpi.com/2218-273X/13/11/1594', event);\">\n    The Role of Soluble Adenylyl Cyclase in the Regulation of Flagellar Motility in Ascidian Sperm.\n  </a>\n  \n  \n  \n</span>\n\n  \n\n</span>\n\n  <i>Biomolecules</i>, 13(11): 1594. October 2023.\n  <span class=\"note\"></span>\n\n<span class=\"bibbase_note\"></span>\n\n<br class=\"bibbase_paper_content\"/>\n\n<span class=\"bibbase_paper_content dontprint\">\n\n  \n  <a href=\"https://www.mdpi.com/2218-273X/13/11/1594\"\n     onclick=\"log_download('shiba-inaba-theroleofsolubleadenylylcyclaseintheregulationofflagellarmotilityinascidiansperm-2023', 'https://www.mdpi.com/2218-273X/13/11/1594', event.ctrlKey, event)\">\n    <img src=\"//bibbase.org/img/filetypes/link.svg\"\n\t     alt=\"The Role of Soluble Adenylyl Cyclase in the Regulation of Flagellar Motility in Ascidian Sperm [link]\"\n       title=\"Paper\"\n\t     class=\"bibbase_icon\"\n         ><span class=\"bibbase_icon_text\">Paper</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"http://doi.org/10.3390/biom13111594\"\n     onclick=\"log_download('shiba-inaba-theroleofsolubleadenylylcyclaseintheregulationofflagellarmotilityinascidiansperm-2023', 'http://doi.org/10.3390/biom13111594', event.ctrlKey)\"\n     class=\"bibbase doi link\">\n    <span>doi</span></a>\n  &nbsp;\n  \n\n  \n  <a href=\"//bibbase.org/network/publication/shiba-inaba-theroleofsolubleadenylylcyclaseintheregulationofflagellarmotilityinascidiansperm-2023\"\n     class=\"bibbase bibbase_page link\">link</a>\n  &nbsp;\n  \n\n  <a href=\"#\"\n     onclick=\"showBib(event); return false;\"\n     class=\"bibbase bibtex link\">bibtex\n    <i class=\"fa fa-caret-down\"></i></a>\n\n  \n  &nbsp;\n  <a class=\"bibbase_abstract_link bibbase link\"\n     href=\"#\"\n     onclick=\"showAbstract(event); return false;\">\n    abstract <i class=\"fa fa-caret-down\"></i></a>\n  \n\n  \n\n  <!-- <a class=\"bibbase read link hidden\" -->\n  <!--    href=\"javascript:toggleRead('shiba-inaba-theroleofsolubleadenylylcyclaseintheregulationofflagellarmotilityinascidiansperm-2023')\" -->\n  <!--    title=\"Marking this paper as read adds it to your library on BibBase.\" -->\n  <!--    > -->\n  <!--   <i class=\"fa fa-check\"></i>&nbsp; -->\n  <!--   <span>mark as read</span> -->\n  <!-- </a> -->\n\n  &nbsp;\n  \n  \n\n</span>\n\n<div class=\"well well-small bibbase\" data-type=\"bibtex\"\n     style=\"display:none\">\n  <pre style=\"white-space: pre-wrap;\">@article{shiba_role_2023,\n\ttitle = {The {Role} of {Soluble} {Adenylyl} {Cyclase} in the {Regulation} of {Flagellar} {Motility} in {Ascidian} {Sperm}},\n\tvolume = {13},\n\tcopyright = {https://creativecommons.org/licenses/by/4.0/},\n\tissn = {2218-273X},\n\turl = {https://www.mdpi.com/2218-273X/13/11/1594},\n\tdoi = {10.3390/biom13111594},\n\tabstract = {Flagellar motility in sperm is activated and regulated by factors related to the eggs at fertilization. In the ascidian Ciona intestinalis, a sulfated steroid called the SAAF (sperm activating and attracting factor) induces both sperm motility activation and chemotaxis. Cyclic AMP (cAMP) is one of the most important intracellular factors in the sperm signaling pathway. Adenylyl cyclase (AC) is the key enzyme that synthesizes cAMP at the onset of the signaling pathway in all cellular functions. We previously reported that both transmembrane AC (tmAC) and soluble AC (sAC) play important roles in sperm motility in Ciona. The tmAC plays a major role in the SAAF-induced activation of sperm motility. On the other hand, sAC is involved in the regulation of flagellar beat frequency and the Ca2+-dependent chemotactic movement of sperm. In this study, we focused on the role of sAC in the regulation of flagellar motility in Ciona sperm chemotaxis. The immunochemical analysis revealed that several isoforms of sAC protein were expressed in Ciona sperm, as reported in mammals and sea urchins. We demonstrated that sAC inhibition caused strong and transient asymmetrization during the chemotactic turn, and then sperm failed to turn toward the SAAF. In addition, real-time Ca2+ imaging in sperm flagella revealed that sAC inhibition induced an excessive and prolonged Ca2+ influx to flagella. These results indicate that sAC plays a key role in sperm chemotaxis by regulating the clearance of [Ca2+]i and by modulating Ca2+-dependent flagellar waveform conversion.},\n\tlanguage = {en},\n\tnumber = {11},\n\turldate = {2024-04-03},\n\tjournal = {Biomolecules},\n\tauthor = {Shiba, Kogiku and Inaba, Kazuo},\n\tmonth = oct,\n\tyear = {2023},\n\tpages = {1594},\n}\n\n</pre>\n</div>\n\n\n<div class=\"well well-small bibbase\" data-type=\"abstract\"\n     style=\"display:none\">\n  Flagellar motility in sperm is activated and regulated by factors related to the eggs at fertilization. In the ascidian Ciona intestinalis, a sulfated steroid called the SAAF (sperm activating and attracting factor) induces both sperm motility activation and chemotaxis. Cyclic AMP (cAMP) is one of the most important intracellular factors in the sperm signaling pathway. Adenylyl cyclase (AC) is the key enzyme that synthesizes cAMP at the onset of the signaling pathway in all cellular functions. We previously reported that both transmembrane AC (tmAC) and soluble AC (sAC) play important roles in sperm motility in Ciona. The tmAC plays a major role in the SAAF-induced activation of sperm motility. On the other hand, sAC is involved in the regulation of flagellar beat frequency and the Ca2+-dependent chemotactic movement of sperm. In this study, we focused on the role of sAC in the regulation of flagellar motility in Ciona sperm chemotaxis. The immunochemical analysis revealed that several isoforms of sAC protein were expressed in Ciona sperm, as reported in mammals and sea urchins. We demonstrated that sAC inhibition caused strong and transient asymmetrization during the chemotactic turn, and then sperm failed to turn toward the SAAF. In addition, real-time Ca2+ imaging in sperm flagella revealed that sAC inhibition induced an excessive and prolonged Ca2+ influx to flagella. These results indicate that sAC plays a key role in sperm chemotaxis by regulating the clearance of [Ca2+]i and by modulating Ca2+-dependent flagellar waveform conversion.\n</div>\n\n\n</div>\n\n\n\n\n\n  </div>\n\n\n  <!-- Modal -->\n\n  <!-- <iframe id=\"bibbase_network_frame\" -->\n  <!--         src=\"//bibbase.org/network/control\" -->\n  <!--         style=\"display: none;\"> -->\n  <!-- </iframe> -->\n\n</div>\n"}; document.write(bibbase_data.data);